Optimizing microarray in experimental hypertension
Shannon, M Frances; McKenzie, Katja; Edgley, Amanda; Rao, Sudha; Peng, Kaimen; Shweta, Amany; Schyvens, Chris; Anderson, Warwick P; Wilson, Susan; Pittelkow, Yvonne; Ohms, Stephen J; Whitworth, Judith
Background. Genetic noise between outbred animals can potentially be a major confounder in the use of microarray technology for gene expression profiling. The study of paired organs from the same animal offers an alternative approach (e.g., for studies of the kidney in experimental hypertension). The present study was undertaken to determine the level of genetic noise between outbred adult Sprague-Dawley (SD) rats, and to determine the effects of unilateral nephrectomy on changes in gene...[Show more]
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