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Type I shorthorn sculpin antifreeze protein. Recombinant synthesis, solution conformation, and ice growth inhibition studies

Fairley, Kayesh; Westman, Belinda J; Pham, Linda H; Haymet, A D J; Harding, Margaret; Mackay, Joel Peter


A number of structurally diverse classes of "antifreeze" proteins that allow fish to survive in sub-zero ice-laden waters have been isolated from the blood plasma of cold water teleosts. However, despite receiving a great deal of attention, the one or more mechanisms through which these proteins act are not fully understood. In this report we have synthesized a type I antifreeze polypeptide (AFP) from the shorthorn sculpin Myoxocephalus scorpius using recombinant methods. Construction of a...[Show more]

CollectionsANU Research Publications
Date published: 2002
Type: Journal article
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.M200307200


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