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Winter Flounder "Antifreeze" Proteins: Synthesis and Ice Growth Inhibition of Analogs that Probe the Relative Importance of Hydrophobic and Hydrogen-Bonding Interactions

Haymet, A D J; Ward, Leanne G; Harding, Margaret

Description

Two series of mutant polypeptides of the type I, 37-residue winter flounder 'antifreeze' protein have been synthesized and analyzed by nanoliter osmometry, the 'ice hemisphere' test, measurement of ice growth hysteresis and circular dichroism (CD) spectroscopy. In series 1 peptides the central two threonines and all four threonines of the native protein were mutated to serine. In series 2 peptides two additional salt bridges (K7, E11 and K29, E33) were incorporated, and all four threonine...[Show more]

CollectionsANU Research Publications
Date published: 1999
Type: Journal article
URI: http://hdl.handle.net/1885/73500
Source: Journal of the American Chemical Society
DOI: 10.1021/ja9801341

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