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Valine substituted winter flounder 'antifreeze': preservation of ice growth hysteresis

Haymet, A D J; Ward, Leanne G; Harding, Margaret; Knight, Charles A


Three mutant polypeptides of the type 1 37-residue winter flounder 'antifreeze' protein have been synthesized. All four threonine residues in the native peptide were been mutated to serine, valine and glycine respectively and two additional salt bridges were incorporated into the sequences in order to improve aqueous solubility. The peptides were analyzed by nanoliter osmometry, the 'ice hemisphere' test, the 'crystal habit' test, measurement of ice growth hysteresis and CD spectroscopy. While...[Show more]

CollectionsANU Research Publications
Date published: 1998
Type: Journal article
Source: FEBS Letters
DOI: 10.1016/s0014-5793(98)00652-8


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