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Use of electrospray ionization mass spectrometry to study binding interactions between a replication terminator protein and DNA

Kapur, Amit; Beck, Jennifer; Brown, Susan Elizabeth; Dixon, Nicholas; Sheil, Margaret


Tus protein binds tightly to specific DNA sequences (Ter) on the Escherichia coli chromosome halting replication. We report here conditions for detecting the 1 : 1 Tus-Ter complex by electrospray ionization mass spectrometry (ESI-MS). ESI mass spectra of

CollectionsANU Research Publications
Date published: 2002
Type: Journal article
Source: Protein Science
DOI: 10.1110/


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