Identification and characterization of GSTT3, a third murine Theta class glutathione transferase
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Coggan, Marjorie; Flanagan, Jack U; Parker, Michael William; Vichai, Vanicha; Pearson, William R; Board, Philip
Description
A novel Theta class glutathione transferase (GST) isoenzyme from mouse termed mGSTT3 has been identified by analysis of the expressed sequence tag database. The gene encoding mGSTT3 is clustered with the mGSTT1 and mGSTT2 genes on chromosome 10 and has an exon/intron structure that is similar to that of the other Theta class genes. mGSTT3 is expressed strongly in the liver and to a decreasing extent in the kidney and testis. Recombinant mGSTT3-3 expressed in Escherichia coli had a...[Show more]
dc.contributor.author | Coggan, Marjorie | |
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dc.contributor.author | Flanagan, Jack U | |
dc.contributor.author | Parker, Michael William | |
dc.contributor.author | Vichai, Vanicha | |
dc.contributor.author | Pearson, William R | |
dc.contributor.author | Board, Philip | |
dc.date.accessioned | 2015-12-13T22:25:15Z | |
dc.date.available | 2015-12-13T22:25:15Z | |
dc.identifier.issn | 0264-6021 | |
dc.identifier.uri | http://hdl.handle.net/1885/73149 | |
dc.description.abstract | A novel Theta class glutathione transferase (GST) isoenzyme from mouse termed mGSTT3 has been identified by analysis of the expressed sequence tag database. The gene encoding mGSTT3 is clustered with the mGSTT1 and mGSTT2 genes on chromosome 10 and has an exon/intron structure that is similar to that of the other Theta class genes. mGSTT3 is expressed strongly in the liver and to a decreasing extent in the kidney and testis. Recombinant mGSTT3-3 expressed in Escherichia coli had a substrate-specificity profile that differed significantly from that of GSTT1-1 and GSTT2-2 isoenzymes. A molecular model of mGSTT3 suggested that, in comparison with GSTT2, a decrease in volume of the hydrophobic substrate-binding site and the loss of the sulphate-binding pocket prevents its use of the GSTT2 substrate 1-menaphthyl sulphate. | |
dc.publisher | Portland Press | |
dc.source | Biochemical Journal | |
dc.subject | Keywords: Biological organs; Escherichia coli; Genes; Hydrophobicity; Substrates; Gene encoding; Enzymes; 1 menaphthyl sulfate; glutathione transferase; glutathione transferase theta; isoenzyme; naphthyl group; unclassified drug; amino acid sequence; article; bindi Database analysis; Enzyme; Glutathione transferase gene | |
dc.title | Identification and characterization of GSTT3, a third murine Theta class glutathione transferase | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 366 | |
dc.date.issued | 2002 | |
local.identifier.absfor | 060107 - Enzymes | |
local.identifier.ariespublication | MigratedxPub3576 | |
local.type.status | Published Version | |
local.contributor.affiliation | Coggan, Marjorie, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Flanagan, Jack U, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Parker, Michael William, St Vincent's Institute, Biota Str Biol Lab | |
local.contributor.affiliation | Vichai, Vanicha, University of Virginia | |
local.contributor.affiliation | Pearson, William R, University of Virginia | |
local.contributor.affiliation | Board, Philip, College of Medicine, Biology and Environment, ANU | |
local.bibliographicCitation.startpage | 323 | |
local.bibliographicCitation.lastpage | 332 | |
local.identifier.doi | 10.1042/BJ20011878 | |
dc.date.updated | 2015-12-11T08:14:21Z | |
local.identifier.scopusID | 2-s2.0-0037103834 | |
Collections | ANU Research Publications |
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