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Gzalpha deficient mice: enzyme levels in the autonomic nervous system, neuronal survival and effect of genetic background

Powell, Kim; Matthaei, Klaus; Heydon, Katharina; Hendry, Ian

Description

Our laboratory has generated a genetically mutant mouse in which the alpha subunit of the heterotrimeric GTP binding protein, Gz has been made dysfunctional by homologous recombination to determine its in vivo function. These animals show a characteristic failure to thrive phenotype. Gzα is expressed in a variety of nervous system tissues as well as in the adrenal medulla. We therefore examined the autonomic nervous system of the Gzα deficient mouse by measuring the activity of tyrosine...[Show more]

dc.contributor.authorPowell, Kim
dc.contributor.authorMatthaei, Klaus
dc.contributor.authorHeydon, Katharina
dc.contributor.authorHendry, Ian
dc.date.accessioned2015-12-13T22:24:52Z
dc.identifier.issn0736-5748
dc.identifier.urihttp://hdl.handle.net/1885/72960
dc.description.abstractOur laboratory has generated a genetically mutant mouse in which the alpha subunit of the heterotrimeric GTP binding protein, Gz has been made dysfunctional by homologous recombination to determine its in vivo function. These animals show a characteristic failure to thrive phenotype. Gzα is expressed in a variety of nervous system tissues as well as in the adrenal medulla. We therefore examined the autonomic nervous system of the Gzα deficient mouse by measuring the activity of tyrosine hydroxylase and choline acetyltransferase in the superior cervical ganglia, submaxillary gland and the adrenal medulla. Preliminary results using animals of mixed BALB/c and C57BL/6 strains gave inconsistent results. Further experiments demonstrated differences in the activity of tyrosine hydroxylase and choline acetyltransferase between BALB/c and C57BL/6 mouse strains. The analysis of the pure strains showed a reduction in the size and enzyme levels of the adrenal gland and submaxillary glands of the Gzα deficient mouse suggesting a role for adrenal insufficiency and/or nutritional disorders for the failure to thrive phenotype. The survival of sympathetic and sensory neurons was also examined in the Gzα deficient mouse and in the presence of pertussis toxin, sympathetic but not sensory neuronal survival in Gzα deficient mice was significantly attenuated. This suggests that in vivo other pertussis toxin sensitive G proteins may be recruited to compensate for the loss of Gzα.
dc.publisherPergamon-Elsevier Ltd
dc.sourceInternational Journal of Developmental Neuroscience
dc.subjectKeywords: brain enzyme; choline acetyltransferase; guanine nucleotide binding protein; pertussis toxin; tyrosine 3 monooxygenase; adrenal insufficiency; adrenal medulla; animal cell; animal experiment; animal tissue; article; autonomic nervous system; cell survival G protein; Genetically mutant mouse; Strain differences
dc.titleGzalpha deficient mice: enzyme levels in the autonomic nervous system, neuronal survival and effect of genetic background
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume20
dc.date.issued2002
local.identifier.absfor060107 - Enzymes
local.identifier.ariespublicationMigratedxPub3501
local.type.statusPublished Version
local.contributor.affiliationPowell, Kim, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMatthaei, Klaus, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHeydon, Katharina, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHendry, Ian, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage39
local.bibliographicCitation.lastpage46
local.identifier.doi10.1016/S0736-5748(02)00002-3
dc.date.updated2015-12-11T08:11:08Z
local.identifier.scopusID2-s2.0-0036242676
CollectionsANU Research Publications

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