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The two substrate-water molecules are already bound to the oxygen evolving complex in the S2 state of photosystem II

Hendry, G; Wydrzynski, Thomas

Description

The first direct evidence which shows that both substrate-water molecules are bound to the O2-evolving catalytic site in the S2 state of photosystem II (PSII) is presented. Rapid 18O isotope exchange measurements between H218O incubated in the S2 state of PSII-enriched membrane samples and the photogenerated O2 reveal a fast and a slow phase of exchange at m/e 34 (which measures the level of the 16O18O product). The rate constant for the slow phase of exchange (34k1) equals 1.9 ± 0.3 s-1 at 10...[Show more]

CollectionsANU Research Publications
Date published: 2002
Type: Journal article
URI: http://hdl.handle.net/1885/72924
Source: Biochemistry
DOI: 10.1021/bi026246t

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