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In situ deprotection and incorporation of unnatural amino acids during cell-free protein synthesis

Arthur, Isaac; Hennessy, James E; Padmakshan, Dharshana; Stigers, Dannon; Lesturgez, Stephanie; Fraser, Samuel; Liutkus, Mantas; Oakeshott , John; Easton, Christopher; Otting, Gottfried


The S30 extract from E. coli BL21 Star (DE3) used for cell-free protein synthesis removes a wide range of α-amino acid protecting groups by cleaving α-carboxyl hydrazides; methyl, benzyl, tert-butyl, and adamantyl esters; tert-butyl and adamantyl carbox

CollectionsANU Research Publications
Date published: 2013
Type: Journal article
Source: Chemistry, A European Journal
DOI: 10.1002/chem.201203923


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