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cDNA cloning of a salivary chymotrypsin-like protease and the identification of six additional cDNAs encoding putative digestive proteases from the green mirid, Creontiades dilutus (Hemiptera: Miridae)

Colebatch, Gillian; Cooper, Paul; East, Peter

Description

RT-PCR with degenerate primers was used to amplify partial cDNA fragments for one serine protease gene and three cysteine protease genes from poly(A) RNA isolated from the midgut of the green mirid, Creontiades dilutus. The serine protease amplicon showed homology to insect trypsin-like protease genes, and all three cysteine protease amplicons showed homology to cathepsin L-like protease genes. RT-PCR was also used to amplify fragments of three serine protease genes from salivary gland poly(A)...[Show more]

CollectionsANU Research Publications
Date published: 2002
Type: Journal article
URI: http://hdl.handle.net/1885/72675
Source: Insect Biochemistry and Molecular Biology
DOI: 10.1016/S0965-1748(02)00044-9

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