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Expression of the Serine/Threonine Kinase hSGK1 in Chronic Viral Hepatitis

Fillon, S; Klingel, Karen; Warntges, Simone; Sauter, Martina; Gabrysch, S; Pestel, Sabine; Tanneur, Valerie; Waldegger, S; Zipfel, Annette; Viebahn, R; Haussinger, D; Broer, Stefan; Kandolf, Reinhart; Lang, Florian

Description

The human serine/threonine kinase hSGK1 is expressed ubiquitously with highest transcript levels in pancreas and liver. This study has been performed to determine the hSGK1 distribution in normal liver and its putative role in fibrosing liver disease. HSGK1-localization was determined by in situ hybridization, regulation of hSGK1-transcription by Northern blotting, fibronectin synthesis and hSGK1 phosphorylation by Western blotting. In normal liver hSGK1 was mainly transcribed by Kupffer cells....[Show more]

dc.contributor.authorFillon, S
dc.contributor.authorKlingel, Karen
dc.contributor.authorWarntges, Simone
dc.contributor.authorSauter, Martina
dc.contributor.authorGabrysch, S
dc.contributor.authorPestel, Sabine
dc.contributor.authorTanneur, Valerie
dc.contributor.authorWaldegger, S
dc.contributor.authorZipfel, Annette
dc.contributor.authorViebahn, R
dc.contributor.authorHaussinger, D
dc.contributor.authorBroer, Stefan
dc.contributor.authorKandolf, Reinhart
dc.contributor.authorLang, Florian
dc.date.accessioned2015-12-13T22:22:39Z
dc.date.available2015-12-13T22:22:39Z
dc.identifier.issn1015-8987
dc.identifier.urihttp://hdl.handle.net/1885/72348
dc.description.abstractThe human serine/threonine kinase hSGK1 is expressed ubiquitously with highest transcript levels in pancreas and liver. This study has been performed to determine the hSGK1 distribution in normal liver and its putative role in fibrosing liver disease. HSGK1-localization was determined by in situ hybridization, regulation of hSGK1-transcription by Northern blotting, fibronectin synthesis and hSGK1 phosphorylation by Western blotting. In normal liver hSGK1 was mainly transcribed by Kupffer cells. In liver tissue from patients with chronic viral hepatitis, hSGK1 transcript levels were excessively high in numerous activated Kupffer cells and inflammatory cells localized within fibrous septum formations. HSGK1 transcripts were also detected in activated hepatic stellate cells. Accordingly, Western blotting revealed that tissue from fibrotic liver expresses excessive hSGK1 protein as compared to normal liver. TGF-β1 (2 ng/ml) increases hSGK1 transcription in both human U937 macrophages and HepG2 hepatoma cells. H2O2 (0.3 mM) activated hSGK1 and increased fibronectin formation in HepG2 cells overexpressing hSGK1 but not in HepG2 cells expressing the inactive mutant hSGK1K127R. In conclusion hSGK1 is upregulated by TGF-β1 during hepatitis and may contribute to enhanced matrix formation during fibrosing liver disease.
dc.publisherS Karger AG
dc.sourceCellular Physiology and Biochemistry
dc.subjectKeywords: cancer growth factor; fibronectin; hydrogen peroxide; phosphotransferase; protein serine kinase; threonine kinase; unclassified drug; article; chronic hepatitis; controlled study; enzyme activation; enzyme activity; enzyme localization; enzyme phosphoryla Cell volume; Fibronectin; HepG2 cells; Liver fibrosis; Macrophages; TGF-ß1
dc.titleExpression of the Serine/Threonine Kinase hSGK1 in Chronic Viral Hepatitis
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume12
dc.date.issued2002
local.identifier.absfor110804 - Medical Virology
local.identifier.ariespublicationMigratedxPub3202
local.type.statusPublished Version
local.contributor.affiliationFillon, S, University of Tubingen
local.contributor.affiliationKlingel, Karen, University of Tubingen
local.contributor.affiliationWarntges, Simone, University of Tubingen
local.contributor.affiliationSauter, Martina, Heinrich Heine University of Dusseldorf
local.contributor.affiliationGabrysch, S, University of Tubingen
local.contributor.affiliationPestel, Sabine, University of Tubingen
local.contributor.affiliationTanneur, Valerie, University of Tubingen
local.contributor.affiliationWaldegger, S, University of Tubingen
local.contributor.affiliationZipfel, Annette, University of Tubingen
local.contributor.affiliationViebahn, R, University of Tubingen
local.contributor.affiliationHaussinger, D, University of Tubingen
local.contributor.affiliationBroer, Stefan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationKandolf, Reinhart, Heinrich Heine University of Dusseldorf
local.contributor.affiliationLang, Florian, University of Tubingen
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage47
local.bibliographicCitation.lastpage54
local.identifier.doi10.1159/000047826
dc.date.updated2015-12-11T07:57:46Z
local.identifier.scopusID2-s2.0-18344377706
CollectionsANU Research Publications

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