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Novel technologies and an overall strategy to allow hazard assessment and risk prediction of chemicals, cosmetics, and drugs with animal-free methods

Leist, Marcel; Lidbury, Brett; Yang, Chihae; Hayden, Patrick J.; Kelm, Jens M; Ringeissen, Stephanie; Detroyer, Ann; Meunier, Jean R; Rathman, James F; Jackson, George R; Stolper, Gina; Hasiwa, Nina

Description

Several alternative methods to replace animal experiments have been accepted by legal bodies. An even larger number of tests are under development or already in use for non-regulatory applications or for the generation of information stored in proprietary knowledge bases. The next step for the use of the different in vitro methods is their combination into integrated testing strategies (ITS) to get closer to the overall goal of predictive "in vitro-based risk evaluation processes." We introduce...[Show more]

dc.contributor.authorLeist, Marcel
dc.contributor.authorLidbury, Brett
dc.contributor.authorYang, Chihae
dc.contributor.authorHayden, Patrick J.
dc.contributor.authorKelm, Jens M
dc.contributor.authorRingeissen, Stephanie
dc.contributor.authorDetroyer, Ann
dc.contributor.authorMeunier, Jean R
dc.contributor.authorRathman, James F
dc.contributor.authorJackson, George R
dc.contributor.authorStolper, Gina
dc.contributor.authorHasiwa, Nina
dc.date.accessioned2015-12-13T22:19:44Z
dc.identifier.issn0946-7785
dc.identifier.urihttp://hdl.handle.net/1885/71973
dc.description.abstractSeveral alternative methods to replace animal experiments have been accepted by legal bodies. An even larger number of tests are under development or already in use for non-regulatory applications or for the generation of information stored in proprietary knowledge bases. The next step for the use of the different in vitro methods is their combination into integrated testing strategies (ITS) to get closer to the overall goal of predictive "in vitro-based risk evaluation processes." We introduce here a conceptual framework as the basis for future ITS and their use for risk evaluation without animal experiments. The framework allows incorporation of both individual tests and already integrated approaches. Illustrative examples for elements to be incorporated are drawn from the session "Innovative technologies" at the 8th World Congress on Alternatives and Animal Use in the Life Sciences, held in Montreal, 2011. For instance, LUHMES cells (conditionally immortalized human neurons) were presented as an example for a 2D cell system. The novel 3D platform developed by InSphero was chosen as an example for the design and use of scaffold-free, organotypic microtissues. The identification of critical pathways of toxicity (PoT) may be facilitated by approaches exemplified by the MatTek 3D model for human epithelial tissues with engineered toxicological reporter functions. The important role of in silico methods and of modeling based on various pre-existing data is demonstrated by Altamira's comprehensive approach to predicting a molecule's potential for skin irritancy. A final example demonstrates how natural variation in human genetics may be overcome using data analytic (pattern recognition) techniques borrowed from computer science and statistics. The overall hazard and risk assessment strategy integrating these different examples has been compiled in a graphical work flow.
dc.publisherSpringer
dc.rightsAuthor/s retain copyright
dc.sourceAltex Alternativen zu Tierexperimenten
dc.subjectKeywords: chemicals and drugs; cosmetic; molecular scaffold; article; automation; biostatistics; cell culture; cell differentiation; cell engineering; computer model; cytotoxicity; decision tree; epithelium; genetic engineering; hazard assessment; human; in vitro s Animal-free methods; Artificial tissue; Risk assessment; Systems toxicology; Tox21
dc.titleNovel technologies and an overall strategy to allow hazard assessment and risk prediction of chemicals, cosmetics, and drugs with animal-free methods
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume29
dc.date.issued2012
local.identifier.absfor111506 - Toxicology (incl. Clinical Toxicology)
local.identifier.absfor060412 - Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
local.identifier.absfor010402 - Biostatistics
local.identifier.ariespublicationf5625xPUB2983
local.type.statusPublished Version
local.contributor.affiliationLeist, Marcel, University of Konstanz
local.contributor.affiliationLidbury, Brett, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationYang, Chihae, Altamira LLC
local.contributor.affiliationHayden, Patrick J, MatTek Corp
local.contributor.affiliationKelm, Jens M, InSphero AG
local.contributor.affiliationRingeissen, Stephanie, L'Oreal Research and Innovation
local.contributor.affiliationDetroyer, Ann, L'Oreal Research and Innovation
local.contributor.affiliationMeunier, Jean R, L'Oreal Research and Innovation
local.contributor.affiliationRathman, James F, Ohio State University
local.contributor.affiliationJackson, George R, MatTek Corp
local.contributor.affiliationStolper, Gina, MatTek Corp
local.contributor.affiliationHasiwa, Nina, University of Konstanz
local.bibliographicCitation.issue4
local.bibliographicCitation.startpage373
local.bibliographicCitation.lastpage388
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.absseo970110 - Expanding Knowledge in Technology
local.identifier.absseo839901 - Animal Welfare
dc.date.updated2016-02-24T09:04:32Z
local.identifier.scopusID2-s2.0-84870984610
local.identifier.thomsonID000311705100003
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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