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Successful whole embryo culture with commercially available reagents

Arkell, Ruth; Glanville-Jones, Hannah; Woo, James


Since its development in the 1970's, whole embryo culture (WEC) has provided an important method of growing and observing murine embryos ex utero. During WEC, embryos are immersed in a combination of rat serum and cell culture media, and supplied with heat and appropriate mixtures of CO2 and oxygen that mimic growth conditions in utero. One significant factor limiting the widespread use of WEC is the perception that commercially produced rat serum is inadequate to support normal rates of...[Show more]

CollectionsANU Research Publications
Date published: 2013
Type: Journal article
Source: International Journal of Developmental Biology
DOI: 10.1387/ijdb.120098ra


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