Analysis of protoplasts and somatic embryogenesis in Medicago truncatula
This thesis examined protoplast proliferation and somatic embryogenesis, by comparing a highly with a poorly embryogenic Medicago truncatula line through microscopic, proteomic and in situ hybridization analysis. Proteome analysis of M. truncatula was used to identify proteins involved in protoplast proliferation and the initiation of somatic embryogenesis. Furthermore, an in situ hybridization study was done to compare the expression of genes known to be involved in zygotic embryogenesis...[Show more]
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