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Connexin37 is major connexin expressed in media of caudal artery

Rummery, Nicole; Hickey, Haruyo; McGurk, Gordon B; Hill, Caryl

Description

Objective - To determine the connexins (Cxs) involved in intercellular coupling within vascular muscle, the present study has quantified mRNA and protein expression for Cx37, Cx40, Cx43, and Cx45 in the caudal artery (CA) and thoracic aorta (ThA) of the rat. Methods and Results - Real-time polymerase chain reaction and immunohistochemistry identified Cx37 as the most abundantly expressed Cx in the CA, with fine punctate staining observed in the media. Conversely, mRNA for Cx43 was 40-fold...[Show more]

dc.contributor.authorRummery, Nicole
dc.contributor.authorHickey, Haruyo
dc.contributor.authorMcGurk, Gordon B
dc.contributor.authorHill, Caryl
dc.date.accessioned2015-12-13T22:17:49Z
dc.identifier.issn1079-5642
dc.identifier.urihttp://hdl.handle.net/1885/71337
dc.description.abstractObjective - To determine the connexins (Cxs) involved in intercellular coupling within vascular muscle, the present study has quantified mRNA and protein expression for Cx37, Cx40, Cx43, and Cx45 in the caudal artery (CA) and thoracic aorta (ThA) of the rat. Methods and Results - Real-time polymerase chain reaction and immunohistochemistry identified Cx37 as the most abundantly expressed Cx in the CA, with fine punctate staining observed in the media. Conversely, mRNA for Cx43 was 40-fold greater in the ThA than in the CA, with punctate staining in the endothelium and media of the ThA but confined to the endothelium in the CA. Western blotting confirmed the differences in the relative amounts of Cx43 between the 2 vessels. For both arteries, Cx45 was expressed to a lesser degree in the media but not in the endothelium, whereas Cx40 was found only in the endothelium. Cx37, Cx40, and Cx43 were expressed in the endothelium of both vessels, although the density of Cx40 plaques was significantly greater in the CA. Conclusions - The demonstration of Cx37 as the dominant Cx in the media of the CA highlights the potential heterogeneity in Cx involvement in vascular smooth muscle.
dc.publisherLippincott Williams & Wilkins
dc.sourceArteriosclerosis Thrombosis and Vascular Biology
dc.subjectKeywords: connexin 37; connexin 40; connexin 43; connexin 45; gap junction protein; messenger RNA; animal model; animal tissue; artery; article; atherosclerotic plaque; controlled study; gene expression; immunohistochemistry; median sacral artery; nonhuman; priorit Caudal artery; Connexin; Endothelium; Smooth muscle; Thoracic aorta
dc.titleConnexin37 is major connexin expressed in media of caudal artery
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume22
dc.date.issued2002
local.identifier.absfor110201 - Cardiology (incl. Cardiovascular Diseases)
local.identifier.ariespublicationMigratedxPub2667
local.type.statusPublished Version
local.contributor.affiliationRummery, Nicole, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHickey, Haruyo, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMcGurk, Gordon B, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHill, Caryl, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue9
local.bibliographicCitation.startpage1427
local.bibliographicCitation.lastpage1432
local.identifier.doi10.1161/01.ATV.0000028814.45706.E5
dc.date.updated2015-12-11T07:36:59Z
local.identifier.scopusID2-s2.0-0036739921
CollectionsANU Research Publications

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