Skip navigation
Skip navigation
The system will be down for maintenance between 8:00 and 8:15am on Thursday 13, December 2018

Role of interleukin-13 in eosinophil accumulation and airway remodelling in a mouse model of chronic asthma

Kumar, Rakesh K; Herbert, John; Yang, Ming; Koskinen, Aulikki; Mckenzie, Andrew N; Foster, Paul S

Description

Background: Interleukin-13 is believed to be important in asthmatic inflammation and airway hyper-reactivity. Objective: To investigate the role of IL-13 in chronic asthma, using an improved experimental model of asthma that reproduces most of the morphological features of the human disease. Methods: BALB/c mice or gene-targeted mice deficient in their ability to produce IL-13 or the IL-4 receptor α-chain (IL-4Rα) were sensitized to ovalbumin and exposed to aerosolized antigen for 30 min/day on...[Show more]

dc.contributor.authorKumar, Rakesh K
dc.contributor.authorHerbert, John
dc.contributor.authorYang, Ming
dc.contributor.authorKoskinen, Aulikki
dc.contributor.authorMckenzie, Andrew N
dc.contributor.authorFoster, Paul S
dc.date.accessioned2015-12-13T22:17:35Z
dc.date.available2015-12-13T22:17:35Z
dc.identifier.issn0954-7894
dc.identifier.urihttp://hdl.handle.net/1885/71227
dc.description.abstractBackground: Interleukin-13 is believed to be important in asthmatic inflammation and airway hyper-reactivity. Objective: To investigate the role of IL-13 in chronic asthma, using an improved experimental model of asthma that reproduces most of the morphological features of the human disease. Methods: BALB/c mice or gene-targeted mice deficient in their ability to produce IL-13 or the IL-4 receptor α-chain (IL-4Rα) were sensitized to ovalbumin and exposed to aerosolized antigen for 30 min/day on 3 days/week for 6 weeks. Intraepithelial eosinophils, accumulation of chronic inflammatory cells in the airway wall, subepithelial fibrosis, epithelial hypertrophy and numbers of mucous cells were quantified histomorphometrically. Airway hyper-reactivity (AHR) to a cholinergic agonist was assessed by barometric plethysmography. Results: Compared with wild-type animals, IL-13-/- mice exhibited diminished accumulation of eosinophils and chronic inflammatory cells, as well as reduced subepithelial fibrosis, epithelial hypertrophy and mucous cell hyperplasia (P < 0.01 for all comparisons). In contrast, AHR was still demonstrable in IL-13-/-mice. In IL-4Rα-/- mice the inflammatory response, subepithelial fibrosis and AHR were similar to wild-type mice, although the receptor-deficient mice had significantly less epithelial hypertrophy and mucous cell hyperplasia. Conclusion: These results imply a critical role for IL-13 in accumulation ofintraepithelial eosinophils in chronic asthma, as well as in epithelial and subepithelial remodelling. In addition, they suggest that in chronic asthma, IL-13 may be capable of signalling via a pathway that does not involve IL-4Rα.
dc.publisherBlackwell Publishing Ltd
dc.sourceClinical and Experimental Allergy
dc.subjectKeywords: interleukin 13; interleukin 4 receptor; interleukin 13; interleukin 4 receptor; airway; animal cell; animal experiment; animal model; animal tissue; article; asthma; bronchus hyperreactivity; cell hyperplasia; controlled study; eosinophil; epithelium; fem Allergy; Cytokines; Eosinophils; In vivo animal models; Inflammation
dc.titleRole of interleukin-13 in eosinophil accumulation and airway remodelling in a mouse model of chronic asthma
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume32
dc.date.issued2002
local.identifier.absfor110203 - Respiratory Diseases
local.identifier.ariespublicationMigratedxPub2617
local.type.statusPublished Version
local.contributor.affiliationKumar, Rakesh K, University of New South Wales
local.contributor.affiliationHerbert, John, University of California
local.contributor.affiliationYang, Ming, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationKoskinen, Aulikki, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMckenzie, Andrew N, Medical Research Council
local.contributor.affiliationFoster, Paul S, College of Medicine, Biology and Environment, ANU
local.bibliographicCitation.startpage1104
local.bibliographicCitation.lastpage1111
local.identifier.doi10.1046/j.1365-2222.2002.01420.x
dc.date.updated2015-12-11T07:35:25Z
local.identifier.scopusID2-s2.0-0035987319
CollectionsANU Research Publications

Download

There are no files associated with this item.


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  27 November 2018/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator