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The Binding of Auxin to the Arabidopsis Auxin Influx Transporter AUX1

Carrier, David J.; Bakar, Norliza Tendot Abu; Napier, Richard M.; Swarup, Ranjan; Bennett, Malcolm J.; Kerr, Ian; Callaghan, Richard

Description

The cellular import of the hormone auxin is a fundamental requirement for the generation of auxin gradients that control a multitude of plant developmental processes. The AUX/LAX family of auxin importers, exemplified by AUX1 from Arabidopsis (Arabidopsis thaliana), has been shown to mediate auxin import when expressed heterologously. The quantitative nature of the interaction between AUX1 and its transport substrate indole-3-acetic acid (IAA) is incompletely understood, and we sought to...[Show more]

dc.contributor.authorCarrier, David J.
dc.contributor.authorBakar, Norliza Tendot Abu
dc.contributor.authorNapier, Richard M.
dc.contributor.authorSwarup, Ranjan
dc.contributor.authorBennett, Malcolm J.
dc.contributor.authorKerr, Ian
dc.contributor.authorCallaghan, Richard
dc.date.accessioned2015-12-13T22:17:28Z
dc.identifier.issn0032-0889
dc.identifier.urihttp://hdl.handle.net/1885/71150
dc.description.abstractThe cellular import of the hormone auxin is a fundamental requirement for the generation of auxin gradients that control a multitude of plant developmental processes. The AUX/LAX family of auxin importers, exemplified by AUX1 from Arabidopsis (Arabidopsis thaliana), has been shown to mediate auxin import when expressed heterologously. The quantitative nature of the interaction between AUX1 and its transport substrate indole-3-acetic acid (IAA) is incompletely understood, and we sought to address this in the present investigation. We expressed AUX1 to high levels in a baculovirus expression system and prepared membrane fragments from baculovirus-infected insect cells. These membranes proved suitable for determination of the binding of IAA to AUX1 and enabled us to determine a Kd of 2.6 μM, comparable with estimates for the Km for IAA transport. The efficacy of a number of auxin analogues and auxin transport inhibitors to displace IAA binding from AUX1 has also been determined and can be rationalized in terms of their physiological effects. Determination of the parameters describing the initial interaction between a plant transporter and its hormone ligand provides novel quantitative data for modeling auxin fluxes.
dc.publisherAmerican Society of Plant Biologists
dc.sourcePlant Physiology
dc.subjectKeywords: Arabidopsis; Arabidopsis thaliana; Hexapoda; Arabidopsis protein; AUX1 protein, Arabidopsis; indoleacetic acid derivative; animal; Arabidopsis; armyworm; article; binding competition; cell line; metabolism; pH; Animals; Arabidopsis; Arabidopsis Proteins;
dc.titleThe Binding of Auxin to the Arabidopsis Auxin Influx Transporter AUX1
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume148
dc.date.issued2008
local.identifier.absfor060199 - Biochemistry and Cell Biology not elsewhere classified
local.identifier.absfor060110 - Receptors and Membrane Biology
local.identifier.absfor060112 - Structural Biology (incl. Macromolecular Modelling)
local.identifier.ariespublicationU3488905xPUB2579
local.type.statusPublished Version
local.contributor.affiliationCarrier, David J., University of Oxford
local.contributor.affiliationBakar, Norliza Tendot Abu, University of Nottingham
local.contributor.affiliationNapier, Richard M., University of Nottingham
local.contributor.affiliationCallaghan, Richard, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSwarup, Ranjan, University of Nottingham
local.contributor.affiliationBennett, Malcolm J., University of Nottingham
local.contributor.affiliationKerr, Ian, University of Nottingham
local.description.embargo2037-12-31
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage529
local.bibliographicCitation.lastpage535
local.identifier.doi10.1104/pp.108.122044
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T10:01:31Z
local.identifier.scopusID2-s2.0-55549109761
CollectionsANU Research Publications

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