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Lanthanide tags for site-specific ligation to an unnatural amino acid and generation of pseudocontact shifts in proteins

Loh, Choy; Ozawa, Kiyoshi; Tuck, Kellie L; Barlow, Nicholas; Graham, Bim; Otting, Gottfried; Huber, Thomas


Pseudocontact shifts (PCS) from paramagnetic lanthanide ions present powerful long-range structural restraints for structural biology by NMR spectroscopy, but site-specific tagging of proteins with lanthanides remains a challenge, as most of the available lanthanide tags require proteins with single cysteine residues. We show that cyclen-based paramagnetic lanthanide tags can be attached to proteins in a site-specific manner by Cu(I)-catalyzed azide-alkyne cycloaddition to a genetically encoded...[Show more]

CollectionsANU Research Publications
Date published: 2013
Type: Journal article
Source: Bioconjugate Chemistry
DOI: 10.1021/bc300631z


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