Twenty-five new polymorphic microsatellites for the eastern mosquitofish, Gambusia holbrooki (Actinopterygii:Poeciliidae), an invasive species in Australia
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Umbers, Kate; Jennions, Michael; Gardner, Michael; Keogh, J Scott
Description
We isolated 25 new polymorphic microsatellite markers from the eastern mosquitofish, Gambusia holbrooki. Initially, 454 shotgun sequencing was used to identify 1187 loci for which primers could be designed. Of these 1187, we trialled 48 in the target species, 40 of which amplified a product of expected size. Subsequently, those 40 loci were screened for variation in 48 individuals from a single population in Canberra, Australia. Twenty loci were in Hardy-Weinberg equilibrium and polymorphic,...[Show more]
dc.contributor.author | Umbers, Kate | |
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dc.contributor.author | Jennions, Michael | |
dc.contributor.author | Gardner, Michael | |
dc.contributor.author | Keogh, J Scott | |
dc.date.accessioned | 2015-12-13T22:15:27Z | |
dc.identifier.issn | 0004-959X | |
dc.identifier.uri | http://hdl.handle.net/1885/70422 | |
dc.description.abstract | We isolated 25 new polymorphic microsatellite markers from the eastern mosquitofish, Gambusia holbrooki. Initially, 454 shotgun sequencing was used to identify 1187 loci for which primers could be designed. Of these 1187, we trialled 48 in the target species, 40 of which amplified a product of expected size. Subsequently, those 40 loci were screened for variation in 48 individuals from a single population in Canberra, Australia. Twenty loci were in Hardy-Weinberg equilibrium and polymorphic, with observed heterozygosity ranging from 0.04 to 0.72 (mean: 0.45±0.18) and the number of alleles per locus ranged from 2 to 5 (mean: 3.20±1.05). These loci will be useful in understanding genetic variation, paternity analysis and in managing this species across both its native and invasive range. | |
dc.publisher | CSIRO Publishing | |
dc.source | Australian Journal of Zoology | |
dc.subject | Keywords: allele; cyprinid; genetic marker; invasive species; native species; polymorphism; population structure; Australia; Australian Capital Territory; Canberra; Actinopterygii; Gambusia holbrooki; Poeciliidae 454 GS-FLX; shotgun sequencing. | |
dc.title | Twenty-five new polymorphic microsatellites for the eastern mosquitofish, Gambusia holbrooki (Actinopterygii:Poeciliidae), an invasive species in Australia | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 60 | |
dc.date.issued | 2012 | |
local.identifier.absfor | 060411 - Population, Ecological and Evolutionary Genetics | |
local.identifier.ariespublication | f5625xPUB2307 | |
local.type.status | Published Version | |
local.contributor.affiliation | Umbers, Kate, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Jennions, Michael, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Gardner, Michael, Flinders University | |
local.contributor.affiliation | Keogh, J Scott, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 4 | |
local.bibliographicCitation.startpage | 235 | |
local.bibliographicCitation.lastpage | 237 | |
local.identifier.doi | 10.1071/ZO12095 | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2016-02-24T08:56:36Z | |
local.identifier.scopusID | 2-s2.0-84872131534 | |
local.identifier.thomsonID | 000313286400003 | |
Collections | ANU Research Publications |
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