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Differences in microRNAs and their expressions between foraging and dancing honey bees, Apis mellifera L.

Li, L; Liu, Fang; Li, Wenfeng; Li, Zhiguo; Pan, J; Yan, Limin; Zhang, Shao Wu; Huang, Zachary Y; Su, Songkun

Description

Many studies have established that microRNAs (miRNAs) regulate gene expression in various biological processes in mammals and insects including honey bees. Dancing behavior is a form of communication unique to honey bees. However, it remains unclear which miRNAs regulate the dancing behavior in honey bees, and how. In the present study, total small RNAs (sRNAs) in Apis mellifera foragers and dancers were extracted and analyzed by a Solexa Sequencer to determine differentially expressed miRNAs....[Show more]

dc.contributor.authorLi, L
dc.contributor.authorLiu, Fang
dc.contributor.authorLi, Wenfeng
dc.contributor.authorLi, Zhiguo
dc.contributor.authorPan, J
dc.contributor.authorYan, Limin
dc.contributor.authorZhang, Shao Wu
dc.contributor.authorHuang, Zachary Y
dc.contributor.authorSu, Songkun
dc.date.accessioned2015-12-10T23:36:51Z
dc.identifier.issn0022-1910
dc.identifier.urihttp://hdl.handle.net/1885/70309
dc.description.abstractMany studies have established that microRNAs (miRNAs) regulate gene expression in various biological processes in mammals and insects including honey bees. Dancing behavior is a form of communication unique to honey bees. However, it remains unclear which miRNAs regulate the dancing behavior in honey bees, and how. In the present study, total small RNAs (sRNAs) in Apis mellifera foragers and dancers were extracted and analyzed by a Solexa Sequencer to determine differentially expressed miRNAs. A small percentage (12.62%) of the unique sRNAs (the number of sequence types) were shared between foragers and dancers, but their expression accounted for 92.92% of the total sRNAs (the number of all sequence reads), and the length of them centered around 22. nt. Out of 58 previously identified miRNAs, 54 were present in both foragers and dancers and most of them were down-regulated in dancers. The fold-changes of ame-miR-34, ame-miR-210, ame-miR-278 and ame-miR-282 were higher than 2. 86 and 104 novel miRNAs were detected in foragers and dancers, respectively. Furthermore, two known miRNAs (ame-miR-278 and ame-miR-282) were confirmed, by qPCR, to have lower expressions in dancers. The target genes of ame-miR-278 and ame-miR-282 were associated with kinase, neural function, synaptotagmin and energy. These results indicate that miRNAs are substantially different between the foraging and dancing stages, and suggest that miRNAs might play important roles in regulating dancing behaviors in honey bees.
dc.publisherElsevier
dc.sourceJournal of Insect Physiology
dc.subjectKeywords: microRNA; behavioral ecology; communication behavior; developmental biology; foraging behavior; gene expression; honeybee; mammal; polymerase chain reaction; RNA; animal; animal communication; appetite; article; bee; comparative study; metabolism; polymer Apis mellifera ligustica; Dancing behavior; Foraging behavior; MicroRNAs
dc.titleDifferences in microRNAs and their expressions between foraging and dancing honey bees, Apis mellifera L.
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume58
dc.date.issued2012
local.identifier.absfor060600 - PHYSIOLOGY
local.identifier.ariespublicationf5625xPUB2285
local.type.statusPublished Version
local.contributor.affiliationLi, L, Zhejiang University
local.contributor.affiliationLiu, Fang, Zhejiang University
local.contributor.affiliationLi, Wenfeng, Zhejiang University
local.contributor.affiliationLi, Zhiguo, Zhejiang University
local.contributor.affiliationPan, J, Zhejiang University
local.contributor.affiliationYan, Limin, Zheijiang University
local.contributor.affiliationZhang, Shao Wu, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHuang, Zachary Y, Michigan State University
local.contributor.affiliationSu, Songkun, Zhejiang University
local.description.embargo2037-12-31
local.bibliographicCitation.issue11
local.bibliographicCitation.startpage1438
local.bibliographicCitation.lastpage1443
local.identifier.doi10.1016/j.jinsphys.2012.08.008
dc.date.updated2016-02-24T08:56:26Z
local.identifier.scopusID2-s2.0-84867889736
local.identifier.thomsonID000310660900008
CollectionsANU Research Publications

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