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De novo metagenomic assembly reveals abundant novel major lineage of Archaea in hypersaline microbial communities

Narasingarao, Priya; Podell, Sheila; Ugalde, Juan A; Brochier-Armanet, Celine; Emerson, Joanne B; Brocks, Jochen; Heidelberg, Karla B; Banfield, Jillian F; Allen, Eric E.


This study describes reconstruction of two highly unusual archaeal genomes by de novo metagenomic assembly of multiple, deeply sequenced libraries from surface waters of Lake Tyrrell (LT), a hypersaline lake in NW Victoria, Australia. Lineage-specific probes were designed using the assembled genomes to visualize these novel archaea, which were highly abundant in the 0.1-0.8 m size fraction of lake water samples. Gene content and inferred metabolic capabilities were highly dissimilar to all...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
Source: The International Society of Microbial Ecology
DOI: 10.1038/ismej.2011.78


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