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Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme

Tokuriki, Nobuhiko; Jackson, Colin; Afriat-Jurnou, Livnat; Wyganowski, Kirsten T; Tang, Renmei; Tawfik, Dan S


Optimization processes, such as evolution, are constrained by diminishing returns - the closer the optimum, the smaller the benefit per mutation, and by tradeoffs - improvement of one property at the cost of others. However, the magnitude and molecular basis of these parameters, and their effect on evolutionary transitions, remain unknown. Here we pursue a complete functional transition of an enzyme with a >109-fold change in the enzyme's selectivity using laboratory evolution. We observed...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
Source: Nature Communications
DOI: 10.1038/ncomms2246
Access Rights: Open Access


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