Hofmeister challenges: Ion binding and charge of the BSA protein as explicit examples
Experiments on bovine serum albumin (BSA) via potentiometric titration (PT) and electrophoretic light scattering (ELS) are used to study specific-ion binding. The effect is appreciable at a physiological concentration of 0.1 M. We found that anions bind to the protein surface at an acidic pH, where the protein carries a positive charge (Zp > 0), according to a Hofmeister series (Cl- < Br- < NO3- < I- < SCN-), as well as at the isoionic point (Zp = 0). The results obtained require critical...[Show more]
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