Skip navigation
Skip navigation

Hofmeister challenges: Ion binding and charge of the BSA protein as explicit examples

Medda, Luca; Barse, Brajesh; Cugia, Francesca; Bostrom, Matias; Parsons, Drew; Ninham, Barry; Monduzzi, Maura; Salis, Andrea

Description

Experiments on bovine serum albumin (BSA) via potentiometric titration (PT) and electrophoretic light scattering (ELS) are used to study specific-ion binding. The effect is appreciable at a physiological concentration of 0.1 M. We found that anions bind to the protein surface at an acidic pH, where the protein carries a positive charge (Zp > 0), according to a Hofmeister series (Cl- < Br- < NO3- < I- < SCN-), as well as at the isoionic point (Zp = 0). The results obtained require critical...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
URI: http://hdl.handle.net/1885/69429
Source: Langmuir
DOI: 10.1021/la3035984

Download

File Description SizeFormat Image
01_Medda_Hofmeister_challenges:_Ion_2012.pdf1.39 MBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  20 July 2017/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator