Medda, Luca; Barse, Brajesh; Cugia, Francesca; Bostrom, Matias; Parsons, Drew; Ninham, Barry; Monduzzi, Maura; Salis, Andrea
Experiments on bovine serum albumin (BSA) via potentiometric titration (PT) and electrophoretic light scattering (ELS) are used to study specific-ion binding. The effect is appreciable at a physiological concentration of 0.1 M. We found that anions bind to the protein surface at an acidic pH, where the protein carries a positive charge (Zp > 0), according to a Hofmeister series (Cl- < Br- < NO3- < I- < SCN-), as well as at the isoionic point (Zp = 0). The results obtained require critical...[Show more]
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