Adsorption of Lysozyme on Hyaluronic Acid Functionalized SBA-15 Mesoporous Silica: A Possible Bioadhesive Depot System
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Medda, Luca; Casula, Maria F; Monduzzi, Maura; Salis, Andrea
Description
Silica-based ordered mesoporous materials are very attractive matrices to prepare smart depot systems for several kinds of therapeutic agents. This work focuses on the well-known SBA-15 mesoporous silica and lysozyme, an antimicrobial protein. In order to improve the bioadhesion properties of SBA-15 particles, the effect of hyaluronic acid (HA) functionalization on lysozyme adsorption was investigated. SBA-15 samples having high (H-SBA) and low (L-SBA) levels of functionalization were analyzed...[Show more]
dc.contributor.author | Medda, Luca | |
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dc.contributor.author | Casula, Maria F | |
dc.contributor.author | Monduzzi, Maura | |
dc.contributor.author | Salis, Andrea | |
dc.date.accessioned | 2015-12-10T23:33:26Z | |
dc.identifier.issn | 0743-7463 | |
dc.identifier.uri | http://hdl.handle.net/1885/69325 | |
dc.description.abstract | Silica-based ordered mesoporous materials are very attractive matrices to prepare smart depot systems for several kinds of therapeutic agents. This work focuses on the well-known SBA-15 mesoporous silica and lysozyme, an antimicrobial protein. In order to improve the bioadhesion properties of SBA-15 particles, the effect of hyaluronic acid (HA) functionalization on lysozyme adsorption was investigated. SBA-15 samples having high (H-SBA) and low (L-SBA) levels of functionalization were analyzed during the three steps of the preparations: (1) introduction of the NH2 groups to obtain the SBA-NH2 samples; (2) functionalization with HA to obtain the SBA-HA matrices; (3) adsorption of lysozyme. All silica matrices were characterized through N2-adsorption/desorption isotherms, small-angle X-ray scattering, transmission electron microscopy, thermogravimetric analysis, and Fourier transform infrared spectroscopy. The whole of the experimental data suggests that a high level of functionalization of the silica surface allows for a negligible lysozyme adsorption mainly due to unfavorable electrostatic interactions (H-SBA-NH2) or steric hindrance (H-SBA-HA). A low degree of functionalization of the silica surface brings about a very good performance toward lysozyme adsorption, being 71% (L-SBA-NH2) and 63% (L-SBA-HA) respectively, compared to that observed for original SBA-15. Finally, two different kinetic models - pseudo-second order and a intraparticle diffusion - were compared to fit lysozyme adsorption data, the latter being more reliable than the former. | |
dc.publisher | American Chemical Society | |
dc.source | Langmuir | |
dc.title | Adsorption of Lysozyme on Hyaluronic Acid Functionalized SBA-15 Mesoporous Silica: A Possible Bioadhesive Depot System | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 30 | |
dc.date.issued | 2014 | |
local.identifier.absfor | 010299 - Applied Mathematics not elsewhere classified | |
local.identifier.ariespublication | a383154xPUB1991 | |
local.type.status | Published Version | |
local.contributor.affiliation | Medda, Luca, University of Cagliari-CNBS and CSGI | |
local.contributor.affiliation | Casula, Maria F, University of Cagliari-CSGI and CNBS | |
local.contributor.affiliation | Monduzzi, Maura, Universita di Cagliari | |
local.contributor.affiliation | Salis, Andrea, College of Physical and Mathematical Sciences, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 12996 | |
local.bibliographicCitation.lastpage | 13004 | |
local.identifier.doi | 10.1021/la503224n | |
dc.date.updated | 2015-12-10T11:29:14Z | |
local.identifier.scopusID | 2-s2.0-84908565228 | |
Collections | ANU Research Publications |
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