Cortical microtubules optimize cell-wall crystallinity to drive unidirectional growth in Arabidopsis
-
Altmetric Citations
Fujita , Miki; Himmelspach, Regina; Hocart, Charles; Williamson, Richard; Mansfield, Shawn; Wasteneys, Geoffrey O
Description
The shape of plants depends on cellulose, a biopolymer that self-assembles into crystalline, inextensible microfibrils (CMFs) upon synthesis at the plasma membrane by multi-enzyme cellulose synthase complexes (CSCs). CSCs are displaced in directions predicted by underlying parallel arrays of cortical microtubules, but CMFs remain transverse in cells that have lost the ability to expand unidirectionally as a result of disrupted microtubules. These conflicting findings suggest that microtubules...[Show more]
dc.contributor.author | Fujita , Miki | |
---|---|---|
dc.contributor.author | Himmelspach, Regina | |
dc.contributor.author | Hocart, Charles | |
dc.contributor.author | Williamson, Richard | |
dc.contributor.author | Mansfield, Shawn | |
dc.contributor.author | Wasteneys, Geoffrey O | |
dc.date.accessioned | 2015-12-10T23:32:25Z | |
dc.identifier.issn | 0960-7412 | |
dc.identifier.uri | http://hdl.handle.net/1885/68834 | |
dc.description.abstract | The shape of plants depends on cellulose, a biopolymer that self-assembles into crystalline, inextensible microfibrils (CMFs) upon synthesis at the plasma membrane by multi-enzyme cellulose synthase complexes (CSCs). CSCs are displaced in directions predicted by underlying parallel arrays of cortical microtubules, but CMFs remain transverse in cells that have lost the ability to expand unidirectionally as a result of disrupted microtubules. These conflicting findings suggest that microtubules are important for some physico-chemical property of cellulose that maintains wall integrity. Using X-ray diffraction, we demonstrate that abundant microtubules enable a decrease in the degree of wall crystallinity during rapid growth at high temperatures. Reduced microtubule polymer mass in the mor1-1 mutant at high temperatures is associated with failure of crystallinity to decrease and a loss of unidirectional expansion. Promotion of microtubule bundling by over-expressing the RIC1 microtubule-associated protein reduced the degree of crystallinity. Using live-cell imaging, we detected an increase in the proportion of CSCs that track in microtubule-free domains in mor1-1, and an increase in the CSC velocity. These results suggest that microtubule domains affect glucan chain crystallization during unidirectional cell expansion. Microtubule disruption had no obvious effect on the orientation of CMFs in dark-grown hypocotyl cells. CMFs at the outer face of the hypocotyl epidermal cells had highly variable orientation, in contrast to the transverse CMFs on the radial and inner periclinal walls. This suggests that the outer epidermal mechanical properties are relatively isotropic, and that axial expansion is largely dependent on the inner tissue layers. | |
dc.publisher | Blackwell Publishing Ltd | |
dc.source | The Plant Journal | |
dc.subject | Keywords: Cell expansion; cell wall; cellulose microfibrils; cellulose synthase complex; Crystallinities; Microtubules; Cell membranes; Cellulose; Chemical properties; Expansion; Mechanical properties; Plants (botany); Tissue; X ray diffraction; Biomechanics; Arabi cell expansion; cell wall; cell-wall crystallinity; cellulose microfibrils; cellulose synthase complex; microtubules | |
dc.title | Cortical microtubules optimize cell-wall crystallinity to drive unidirectional growth in Arabidopsis | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 66 | |
dc.date.issued | 2011 | |
local.identifier.absfor | 060705 - Plant Physiology | |
local.identifier.ariespublication | f2965xPUB1843 | |
local.type.status | Published Version | |
local.contributor.affiliation | Fujita , Miki, University of British Columbia | |
local.contributor.affiliation | Himmelspach, Regina, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Hocart, Charles, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Williamson, Richard, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Mansfield, Shawn, University of British Columbia | |
local.contributor.affiliation | Wasteneys, Geoffrey O, University of British Columbia | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 6 | |
local.bibliographicCitation.startpage | 915 | |
local.bibliographicCitation.lastpage | 928 | |
local.identifier.doi | 10.1111/j.1365-313X.2011.04552.x | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2016-02-24T08:18:28Z | |
local.identifier.scopusID | 2-s2.0-79958231688 | |
local.identifier.thomsonID | 000292103900001 | |
Collections | ANU Research Publications |
Download
File | Description | Size | Format | Image |
---|---|---|---|---|
01_Fujita _Cortical_microtubules_optimize_2011.pdf | 1.58 MB | Adobe PDF | Request a copy |
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.
Updated: 17 November 2022/ Responsible Officer: University Librarian/ Page Contact: Library Systems & Web Coordinator