Breeding system, genetic diversity and clonal structure in the sub-alpine forb Rutidosis leiolepis F. Muell. (Asteraceae)

Abstract

Genetic markers, controlled pollinations and chromosome analysis were used to examine the breeding system, clonal structure, genetic diversity and cytological complexity of populations of the endangered sub-alpine forb Rutidosis leiolepis. Results show that R. leiolepis: (1) has a strong sporophytic self-incompatibility system; (2) exhibits significant clonality and that the importance of vegetative reproduction increases at higher altitudes; (3) is genetically diverse, but that variation within populations decreases and differentiation among populations increases with altitude; and (4) is diploid with 2n = 22 throughout its geographic range. Based on these results, low altitude populations around Cooma and Happy Valley should be made priority areas for in situ conservation in the Species Recovery Plan currently being developed by the New South Wales National Parks and Wildlife Service because they exhibit the most sexual reproduction and highest levels of genetic variation within the species. They may also be demographically more viable in the long term, having larger effective population sizes and less likelihood of mate limitation due to low S allele richness than high altitude populations. Sampling strategies for ex situ conservation should vary from large collections within populations at low altitudes to smaller collections spread across populations at higher altitudes where more of the genetic diversity is partitioned among sites. This should give maximum representation of the species' gene pool for minimum sampling effort.