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Quantification of Rubisco activase content in leaf extracts

Yamori, Wataru; von Caemmerer, Susanne

Description

Rubisco activase functions to promote and maintain the catalytic activity of Rubisco. Studies with the activase-lacking Arabidopsis rca mutant (Salvucci et al. Photosynth Res 7:193-201, 1985; Salvucci et al. Plant Physiol 80:655-659, 1986), antisense activase tobacco, Arabidopsis and Flaveria bidentis plants (Mate et al. Plant Physiol 102:1119-1128, 1993; Eckardt et al. Plant Physiol 113:575-586, 1997; von Caemmerer et al. Plant Physiol 137:747-755, 2005) have shown that photosynthesis at...[Show more]

dc.contributor.authorYamori, Wataru
dc.contributor.authorvon Caemmerer, Susanne
dc.date.accessioned2015-12-10T23:26:31Z
dc.identifier.isbn9781607619246
dc.identifier.urihttp://hdl.handle.net/1885/67800
dc.description.abstractRubisco activase functions to promote and maintain the catalytic activity of Rubisco. Studies with the activase-lacking Arabidopsis rca mutant (Salvucci et al. Photosynth Res 7:193-201, 1985; Salvucci et al. Plant Physiol 80:655-659, 1986), antisense activase tobacco, Arabidopsis and Flaveria bidentis plants (Mate et al. Plant Physiol 102:1119-1128, 1993; Eckardt et al. Plant Physiol 113:575-586, 1997; von Caemmerer et al. Plant Physiol 137:747-755, 2005) have shown that photosynthesis at atmospheric levels of CO2 is severely impaired when plants lack activase because Rubisco becomes sequestered in an inactive form. Activase protein has been detected in all plant species, including C3 and C4 plants and green algae (Salvucci et al. Plant Physiol 84:930-936, 1987). Rubisco activase is essential in all these photosynthetic organisms for photosynthesis and plant growth. The physiological importance of Rubisco activase is reinforced by recent studies indicating that it plays a role in the response of photosynthesis to temperature. In this chapter, we describe how to extract and quantify Rubisco activase content in leaf.
dc.publisherSpringer
dc.relation.ispartofPhotosynthesis Research Protocols: Methods in Molecular Biology (2nd ed)
dc.relation.isversionof2nd Edition
dc.subjectKeywords: plant extract; pyroxylin; rca protein, plant; vegetable protein; article; artificial membrane; chemistry; enzymology; fractionation; immunoassay; isolation and purification; metabolism; plant leaf; polyacrylamide gel electrophoresis; solubility; Western b
dc.titleQuantification of Rubisco activase content in leaf extracts
dc.typeBook chapter
local.description.notesImported from ARIES
dc.date.issued2011
local.identifier.absfor060702 - Plant Cell and Molecular Biology
local.identifier.ariespublicationf2965xPUB1527
local.type.statusPublished Version
local.contributor.affiliationYamori, Wataru, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationvon Caemmerer, Susanne, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage383
local.bibliographicCitation.lastpage391
local.identifier.doi10.1007/978-1-60761-925-3_30
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T08:14:52Z
local.bibliographicCitation.placeofpublicationNew York
local.identifier.scopusID2-s2.0-79952118205
CollectionsANU Research Publications

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