Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enzymes for selective 15 N-labelling and production of perdeuterated proteins in H 2 O
Selectively isotope labelled protein samples can be prepared in vivo or in vitro from selectively labeled amino acids but, in many cases, metabolic conversions between different amino acids result in isotope scrambling. The best results are obtained by cell-free protein synthesis, where metabolic enzymes are generally less active, but isotope scrambling can never be suppressed completely. We show that reduction of E. coli S30 extracts with NaBH4 presents a simple and inexpensive way to achieve...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of Biomolecular NMR|
|01_Su_Suppression_of_isotope_2011.pdf||539.59 kB||Adobe PDF||Request a copy|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.