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Ice barriers promote supercooling and prevent frost injury in reproductive buds, flowers and fruits of alpine dwarf shrubs throughout the summer

Kuprian, Edith; Briceno, Veronica; Wagner, Johanna; Neuner, Gilbert

Description

Over-wintering reproductive buds of many woody plants survive frost by supercooling. The bud tissues are isolated from acropetally advancing ice by the presence of ice barriers that restrict ice growth. Plants living in alpine environments also face the risk of ice formation in summer months. Little knowledge exists, how reproductive structures of woody alpine plants are protected from frost injury during episodic summer frosts. In order to address this question, frost resistance of three...[Show more]

dc.contributor.authorKuprian, Edith
dc.contributor.authorBriceno, Veronica
dc.contributor.authorWagner, Johanna
dc.contributor.authorNeuner, Gilbert
dc.date.accessioned2015-12-10T23:24:02Z
dc.identifier.issn0098-8472
dc.identifier.urihttp://hdl.handle.net/1885/67066
dc.description.abstractOver-wintering reproductive buds of many woody plants survive frost by supercooling. The bud tissues are isolated from acropetally advancing ice by the presence of ice barriers that restrict ice growth. Plants living in alpine environments also face the risk of ice formation in summer months. Little knowledge exists, how reproductive structures of woody alpine plants are protected from frost injury during episodic summer frosts. In order to address this question, frost resistance of three common dwarf shrubs, Calluna vulgaris, Empetrum hermaphroditum and Loiseleuria procumbens was measured and ice formation and propagation were monitored in twigs bearing reproductive shoots during various stages of reproductive development (bud, anthesis, and fruit) throughout the alpine summer. Results indicated that, in the investigated species, ice barriers were present at all reproductive stages, isolating the reproductive shoots from ice advancing from the subtending vegetative shoot. Additionally, in the reproductive stems ice nucleating agents that are active at warm, sub-zero temperatures, were absent. The ice barriers were 100% effective, with the exception of L. procumbens, where in 13% of the total observations, the ice barrier failed. The ice barriers were localized at the base of the pedicel, at the anatomical junction of the vegetative and reproductive shoot. There, structural aspects of the tissue impede or prevent ice from advancing from the frozen stem into the pedicel of the reproductive shoot. Under the experimental conditions used in this study, ice nucleation initially occurred in the stem of the vegetative shoot at species-specific mean temperatures in the range of -4.7 to -5.8. °C. Reproductive shoots, however, remained supercooled and ice free down to a range of -7.2 to -18.2. °C or even below -22. °C, the lowest temperature applied in the study. This level of supercooling is sufficient to prevent freezing of reproductive structures at the lowest air temperature occurring at the altitude of the upper distribution boundary of the natural habitat of the investigated species which is between -8 and -10. °C in summer. Frost resistance assays indicated that reproductive shoots are much less frost resistant than vegetative stems, and in contrast to vegetative shoots, are not ice tolerant. Supercooling of reproductive shoots in alpine, woody plant species is an effective mechanism that protects developing offspring from potential frost damage resulting from episodic summer freezing events.
dc.publisherElsevier
dc.sourceEnvironmental and Experimental Botany
dc.titleIce barriers promote supercooling and prevent frost injury in reproductive buds, flowers and fruits of alpine dwarf shrubs throughout the summer
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume106
dc.date.issued2014
local.identifier.absfor060799 - Plant Biology not elsewhere classified
local.identifier.ariespublicationu9511635xPUB1390
local.type.statusPublished Version
local.contributor.affiliationKuprian, Edith, University of Innsbruck
local.contributor.affiliationBriceno, Veronica, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWagner, Johanna, University of Innsbruck
local.contributor.affiliationNeuner, Gilbert, University of Innsbruck
local.description.embargo2037-12-31
local.bibliographicCitation.startpage4
local.bibliographicCitation.lastpage12
local.identifier.doi10.1016/j.envexpbot.2014.01.011
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2015-12-10T10:43:43Z
local.identifier.scopusID2-s2.0-84903883225
local.identifier.thomsonID000340315800002
CollectionsANU Research Publications

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