Microarray analysis of gene expression in West Nile virus-infected human retinal pigment epithelium
Purpose: To identify key genes differentially expressed in the human retinal pigment epithelium (hRPE) following lowlevel West Nile virus (WNV) infection. Methods: Primary hRPE and retinal pigment epithelium cell line (ARPE-19) cells were infected with WNV (multiplicity of infection 1). RNA extracted from mock-infected and WNV-infected cells was assessed for differential expression of genes using Affymetrix microarray. Quantitative real-time PCR analysis of 23 genes was used to validate the...[Show more]
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