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Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing

Clermont, Olivier; Christenson, Julia; Daubie, Anne-Sophie; Gordon, David; Denamur, Erick

Description

We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
URI: http://hdl.handle.net/1885/66137
Source: Journal of Microbiological Methods
DOI: 10.1016/j.mimet.2014.03.008

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