Skip navigation
Skip navigation

Engineering recombinant poxviruses using a compact GFP-blasticidin resistance fusion gene for selection

Wong, Yik Chun; Lin, Chien-Wei (Leon); Silva, Carolina; Smith, Stewart; Tscharke, David


Recombinant poxviruses are important tools for research and some are candidate vaccines. To make these viruses a simple, small vector that can be used to engineer multiple strains of vaccinia virus and other model poxviruses, including ectromelia virus is of value. Here a set of plasmids and methods for making these viruses that uses an enhanced green fluorescent protein-blasticidin resistance (GFP-bsd) fusion gene as a transient selectable marker are described. This gene is smaller than any of...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
Source: Journal of Virological Methods
DOI: 10.1016/j.jviromet.2010.11.003


File Description SizeFormat Image
01_Wong_Engineering_recombinant_2011.pdf255.44 kBAdobe PDF    Request a copy

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator