Engineering recombinant poxviruses using a compact GFP-blasticidin resistance fusion gene for selection
Recombinant poxviruses are important tools for research and some are candidate vaccines. To make these viruses a simple, small vector that can be used to engineer multiple strains of vaccinia virus and other model poxviruses, including ectromelia virus is of value. Here a set of plasmids and methods for making these viruses that uses an enhanced green fluorescent protein-blasticidin resistance (GFP-bsd) fusion gene as a transient selectable marker are described. This gene is smaller than any of...[Show more]
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|Source:||Journal of Virological Methods|
|01_Wong_Engineering_recombinant_2011.pdf||255.44 kB||Adobe PDF||Request a copy|
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