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Engineering recombinant poxviruses using a compact GFP-blasticidin resistance fusion gene for selection

Wong, Yik Chun; Lin, Chien-Wei (Leon); Silva, Carolina; Smith, Stewart; Tscharke, David

Description

Recombinant poxviruses are important tools for research and some are candidate vaccines. To make these viruses a simple, small vector that can be used to engineer multiple strains of vaccinia virus and other model poxviruses, including ectromelia virus is of value. Here a set of plasmids and methods for making these viruses that uses an enhanced green fluorescent protein-blasticidin resistance (GFP-bsd) fusion gene as a transient selectable marker are described. This gene is smaller than any of...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
URI: http://hdl.handle.net/1885/65393
Source: Journal of Virological Methods
DOI: 10.1016/j.jviromet.2010.11.003

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