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Proline residues in two tightly coupled helices of the sulphate transporter, SHST1, are important for sulphate transport

Shelden, Megan; Loughlin, Patrick; Tierney, Mary (Louise); Howitt, Susan


The sulphate transporter SHST1, from Stylosanthes hamata, features three tightly coupled transmembrane helices which include proline residues that are conserved in most related transporters. We used site-directed mutagenesis and expression of the mutant transporters in yeast to test whether these proline residues are important for function. Four proline residues were replaced by both alanine and leucine. Only one of these proline residues, Pro-144, was essential for sulphate transport. However,...[Show more]

CollectionsANU Research Publications
Date published: 2001
Type: Journal article
Source: Biochemical Journal
DOI: 10.1042/0264-6021:3560589


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