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Directed gene silencing with artificial microRNAs

Schwab, Rebecca; Ossowski, Stephan; Warthmann, Norman; Weigel, Detlef

Description

The characterization of gene function typically includes a detailed analysis of loss-of-function alleles. In model plants, such as Arabidopsis thaliana and rice, sequence-indexed insertion collections provide a large resource of potential null alleles that can often be easily accessed through convenient Web sites (e.g., http://signal.salk.edu ). They are, however, not available for nonmodel species, require stacking for knockout of redundant homologs, and do not easily allow for partial or...[Show more]

CollectionsANU Research Publications
Date published: 2010
Type: Book chapter
URI: http://hdl.handle.net/1885/65209
DOI: 10.1007/978-1-60327-005-2

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