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Directed gene silencing with artificial microRNAs

Schwab, Rebecca; Ossowski, Stephan; Warthmann, Norman; Weigel, Detlef


The characterization of gene function typically includes a detailed analysis of loss-of-function alleles. In model plants, such as Arabidopsis thaliana and rice, sequence-indexed insertion collections provide a large resource of potential null alleles that can often be easily accessed through convenient Web sites (e.g., ). They are, however, not available for nonmodel species, require stacking for knockout of redundant homologs, and do not easily allow for partial or...[Show more]

CollectionsANU Research Publications
Date published: 2010
Type: Book chapter
Book Title: Plant MicroRNA's
DOI: 10.1007/978-1-60327-005-2


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