Inhibition of bacterial RNA polymerase by the cyanobacterial metabolites 12- epi -hapalindole E isonitrile and calothrixin A
The alkaloid 12-epi-hapalindole E isonitrile, from a cyanobacterial Fischerella species, and the indolophenanthridine calothrixin A, from Calothrix, inhibited Escherichia coli RNA polymerase competitively with respect to ATP, and non-competitively with respect to UTP. The inhibition was dependent on the order of addition of the inhibitors. The KI values, with ATP as the variable substrate, were 1.3±0.2 mM and 0.23±0.11 mM, respectively. Based on comparisons with the sensitivity of whole cells...[Show more]
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|Source:||FEMS Microbiology Letters|
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