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Single epitope multiple staining to detect ultralow frequency B cells

Townsend, S E; Goodnow, Christopher; Cornall, Richard J

Description

Here we describe a method for detecting ultralow frequency target cells from within a high background of irrelevant cells by a novel method, single epitope multiple staining (SEMS). Samples of murine splenocytes were seeded with a low number of splenocytes from mice transgenic for a hen eggwhite lysozyme (HEL)-specific immunoglobulin (Ig). These samples were stained with two reagents specific for the same epitope expressed by the transgenic B cells, which had been conjugated to two different...[Show more]

CollectionsANU Research Publications
Date published: 2001
Type: Journal article
URI: http://hdl.handle.net/1885/65005
Source: Journal of Immunological Methods
DOI: 10.1016/S0022-1759(00)00352-5

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