Single epitope multiple staining to detect ultralow frequency B cells
Here we describe a method for detecting ultralow frequency target cells from within a high background of irrelevant cells by a novel method, single epitope multiple staining (SEMS). Samples of murine splenocytes were seeded with a low number of splenocytes from mice transgenic for a hen eggwhite lysozyme (HEL)-specific immunoglobulin (Ig). These samples were stained with two reagents specific for the same epitope expressed by the transgenic B cells, which had been conjugated to two different...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of Immunological Methods|
|01_Townsend_Single_epitope_multiple_2001.pdf||1.19 MB||Adobe PDF||Request a copy|
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