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A new approach for spot detection in total internal reflection fluorescence microscopy

Rezatofighi, Seyed; Hartley, Richard; Hughes, William E

Description

Biological images acquired from fluorescence microscopy-based imaging techniques, such as total internal reflection fluorescence microscopy, generally contain hundreds of subcellular components, appearing in the images as bright spots. Therefore, the first step of analysis of these images usually involves the detection of these bright spots. In this paper, we propose a new approach for spot detection using the h-dome transform along with an adaptive mask obtained from regional information....[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Conference paper
URI: http://hdl.handle.net/1885/64898
Source: Proceedings - International Symposium on Biomedical Imaging
DOI: 10.1109/ISBI.2012.6235684

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