Altering the substrate specificity of methyl parathion hydrolase with directed evolution
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Ng, Tee-Kheang; Gahan, Lawrence; Schenk, Gerhard; Ollis, David
Description
Abstract Many organophosphates (OPs) are used as pesticides in agriculture. They pose a severe health hazard due to their inhibitory effect on acetylcholinesterase. Therefore, detoxification of water and soil contaminated by OPs is important. Metalloenzymes such as methyl parathion hydrolase (MPH) from Pseudomonas sp. WBC-3 hold great promise as bioremediators as they are able to hydrolyze a wide range of OPs. MPH is highly efficient towards methyl parathion (1 × 106 s-1 M-1), but its activity...[Show more]
dc.contributor.author | Ng, Tee-Kheang | |
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dc.contributor.author | Gahan, Lawrence | |
dc.contributor.author | Schenk, Gerhard | |
dc.contributor.author | Ollis, David | |
dc.date.accessioned | 2015-12-10T23:13:13Z | |
dc.identifier.issn | 0003-9861 | |
dc.identifier.uri | http://hdl.handle.net/1885/64325 | |
dc.description.abstract | Abstract Many organophosphates (OPs) are used as pesticides in agriculture. They pose a severe health hazard due to their inhibitory effect on acetylcholinesterase. Therefore, detoxification of water and soil contaminated by OPs is important. Metalloenzymes such as methyl parathion hydrolase (MPH) from Pseudomonas sp. WBC-3 hold great promise as bioremediators as they are able to hydrolyze a wide range of OPs. MPH is highly efficient towards methyl parathion (1 × 106 s-1 M-1), but its activity towards other OPs is more modest. Thus, site saturation mutagenesis (SSM) and DNA shuffling were performed to find mutants with improved activities on ethyl paraxon (6.1 × 103 s-1 M-1). SSM was performed on nine residues lining the active site. Several mutants with modest activity enhancement towards ethyl paraoxon were isolated and used as templates for DNA shuffling. Ultimately, 14 multiple-site mutants with enhanced activity were isolated. One mutant, R2F3, exhibited a nearly 100-fold increase in the kcat/Km value for ethyl paraoxon (5.9 × 105 s-1 M-1). These studies highlight the 'plasticity' of the MPH active site that facilitates the fine-tuning of its active site towards specific substrates with only minor changes required. MPH is thus an ideal candidate for the development of an enzyme-based bioremediation system. | |
dc.publisher | Academic Press | |
dc.source | Archives of Biochemistry and Biophysics | |
dc.title | Altering the substrate specificity of methyl parathion hydrolase with directed evolution | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 573 | |
dc.date.issued | 2015 | |
local.identifier.absfor | 039999 - Chemical Sciences not elsewhere classified | |
local.identifier.ariespublication | u4005981xPUB917 | |
local.type.status | Published Version | |
local.contributor.affiliation | Ng, Tee-Kheang, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Gahan, Lawrence, University of Queensland | |
local.contributor.affiliation | Schenk, Gerhard, University of Queensland | |
local.contributor.affiliation | Ollis, David, College of Physical and Mathematical Sciences, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 59 | |
local.bibliographicCitation.lastpage | 68 | |
local.identifier.doi | 10.1016/j.abb.2015.03.012 | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2015-12-10T09:37:35Z | |
local.identifier.scopusID | 2-s2.0-84925850321 | |
Collections | ANU Research Publications |
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