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Altering the substrate specificity of methyl parathion hydrolase with directed evolution

Ng, Tee-Kheang; Gahan, Lawrence; Schenk, Gerhard; Ollis, David


Abstract Many organophosphates (OPs) are used as pesticides in agriculture. They pose a severe health hazard due to their inhibitory effect on acetylcholinesterase. Therefore, detoxification of water and soil contaminated by OPs is important. Metalloenzymes such as methyl parathion hydrolase (MPH) from Pseudomonas sp. WBC-3 hold great promise as bioremediators as they are able to hydrolyze a wide range of OPs. MPH is highly efficient towards methyl parathion (1 × 106 s-1 M-1), but its activity...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
Source: Archives of Biochemistry and Biophysics
DOI: 10.1016/


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