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Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae)

Sakaguchi, Shota; Uchiyama, Kentaro; Ueno, Saneyoshi; Ujino-Ihara, Tokuko; Tsumura, Yoshihiko; Prior, Lynda D.; Bowman, David M.J.S.; Crisp, Michael; Isagi, Yuji


Premise of the study: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan. Methods and Results: mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing. Two hundred and nineteen primer pairs bordering sequence regions were designed from the obtained sequence data. In total, 52 showed polymorphism...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
Source: American Journal of Botany
DOI: 10.3732/ajb.1100276


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