Sakaguchi, Shota; Uchiyama, Kentaro; Ueno, Saneyoshi; Ujino-Ihara, Tokuko; Tsumura, Yoshihiko; Prior, Lynda D.; Bowman, David M.J.S.; Crisp, Michael; Isagi, Yuji
Premise of the study: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan. Methods and Results: mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing. Two hundred and nineteen primer pairs bordering sequence regions were designed from the obtained sequence data. In total, 52 showed polymorphism...[Show more]
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