New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes
The use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to measure lymphocyte proliferation by flow cytometry has become one of the most widely utilised assays for assessing lymphocyte responses. The properties of CFSE make it ideal for such a task, covalently labelling cells with a long-lived fluorescence of high intensity and low variance with minimal cell toxicity. No dye in the last 20. years has been capable of replicating CFSE in these respects. However, currently CFSE is...[Show more]
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|Source:||Journal of Immunological Methods|
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