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An in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum

Chooi, Yit-Heng; Krill, Christian; Barrow, Russell; Chen, Shasha; Trengove, Robert; Oliver, Richard P.; Solomon, Peter

Description

Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-mellein and (R)-O-methylmellein. Using a Saccharomyces cerevisiae yeast heterologous expression...[Show more]

dc.contributor.authorChooi, Yit-Heng
dc.contributor.authorKrill, Christian
dc.contributor.authorBarrow, Russell
dc.contributor.authorChen, Shasha
dc.contributor.authorTrengove, Robert
dc.contributor.authorOliver, Richard P.
dc.contributor.authorSolomon, Peter
dc.date.accessioned2015-12-10T23:12:02Z
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/1885/63933
dc.description.abstractParastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-mellein and (R)-O-methylmellein. Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-mellein. The P. nodorum ΔSN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-mellein at 200 μg/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera. Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases.
dc.publisherAmerican Society for Microbiology
dc.rightsAuthor/s retain copyright
dc.sourceApplied and Environmental Microbiology
dc.titleAn in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume81
dc.date.issued2015
local.identifier.absfor030502 - Natural Products Chemistry
local.identifier.ariespublicationu4005981xPUB865
local.type.statusPublished Version
local.contributor.affiliationChooi, Yit-Heng, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationKrill, Christian , Murdoch University
local.contributor.affiliationBarrow, Russell, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationChen, Shasha, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationTrengove, Robert, Murdoch University
local.contributor.affiliationOliver , Richard P, Curtin University
local.contributor.affiliationSolomon, Peter, College of Medicine, Biology and Environment, ANU
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage177
local.bibliographicCitation.lastpage186
local.identifier.doi10.1128/AEM.02745-14
local.identifier.absseo970103 - Expanding Knowledge in the Chemical Sciences
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2015-12-10T09:26:01Z
local.identifier.scopusID2-s2.0-84917729581
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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