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Iron(III) located in the dinuclear metallo-β-lactamase IMP-1 by pseudocontact shifts

Carruthers, Thomas; Carr, Paul D; Loh, Choy; Jackson, Colin; Otting, Gottfried

Description

Heterodinuclear metalloenzymes are an important class of metalloproteins, but determining the location of the different metal ions can be difficult. Herein we present a new NMR spectroscopy method that uses pseudocontact shifts (PCS) to achieve this without assumptions about the coordinating ligands. The approach is illustrated with the dinuclear [FeZn] complex of IMP-1, which is a prototypical metallo-β-lactamase (MβL) that confers resistance to β-lactam antibiotics. Results from...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
URI: http://hdl.handle.net/1885/63884
Source: Angewandte Chemie International Edition
DOI: 10.1002/anie.201408693

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