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Determining the oligomeric structure of proteorhodopsin by Gd3+-based pulsed dipolar spectroscopy of multiple distances

Edwards, Devin T.; Huber, Thomas; Hussain, Sunyia; Stone, Katherine M.; Kinnebrew, Maia; Kaminker, Ilia; Matalon, Erez; Sherwin, Mark S.; Goldfarb, Daniella; Han, Songi

Description

The structural organization of the functionally relevant, hexameric oligomer of green-absorbing proteorhodopsin (G-PR) was obtained from double electron-electron resonance (DEER) spectroscopy utilizing conventional nitroxide spin labels and recently developed Gd3+-based spin labels. G-PR with nitroxide or Gd3+ labels was prepared using cysteine mutations at residues Trp58 and Thr177. By combining reliable measurements of multiple interprotein distances in the G-PR hexamer with computer...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
URI: http://hdl.handle.net/1885/63783
Source: Structure
DOI: 10.1016/j.str.2014.09.008

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