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300-Fold Increase in Production of the Zn 2+ -Dependent Dechlorinase TrzN in Soluble Form via Apoenzyme Stabilization

Jackson, Colin; Coppin, Christopher W; Carr, Paul D; Aleksandrov, Alexey; Wilding, Matthew; Sugrue, Elena; Ubels, Joanna; Paks, Michael; Newman, Janet; Peat, Thomas S; Russell, Robyn J; Field, Martin; Weik, Martin; Oakeshott , John; Scott, Colin


Microbial metalloenzymes constitute a large library of biocatalysts, a number of which have already been shown to catalyze the breakdown of toxic chemicals or industrially relevant chemical transformations. However, while there is considerable interest in harnessing these catalysts for biotechnology, for many of the enzymes, their large-scale production in active, soluble form in recombinant systems is a significant barrier to their use. In this work, we demonstrate that as few as three...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
Source: Applied and Environmental Microbiology
DOI: 10.1128/AEM.00916-14
Access Rights: Open Access


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