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Alternatively activated macrophage possess antitumor cytotoxicity that is induced by il-4 and mediated by arginase-1

Ellyard, Julia; Quah, Ben; Simson, Ljubov; Parish, Christopher

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Earlier studies have shown that the adoptive transfer of Th2-polarized CD4+ T cells can clear established tumors from mice in an antigen-specific manner. Although eosinophils were implicated in this process, the exact mechanism of tumor clearance and which immune effector cells were involved, remain to be defined. Consequently, experiments were undertaken to elucidate the mechanism of Th2-mediated destruction of B16-F1 melanoma cells by examining the in vitro antitumor activity of leukocytes...[Show more]

dc.contributor.authorEllyard, Julia
dc.contributor.authorQuah, Ben
dc.contributor.authorSimson, Ljubov
dc.contributor.authorParish, Christopher
dc.date.accessioned2015-12-10T23:09:56Z
dc.identifier.issn1524-9557
dc.identifier.urihttp://hdl.handle.net/1885/63504
dc.description.abstractEarlier studies have shown that the adoptive transfer of Th2-polarized CD4+ T cells can clear established tumors from mice in an antigen-specific manner. Although eosinophils were implicated in this process, the exact mechanism of tumor clearance and which immune effector cells were involved, remain to be defined. Consequently, experiments were undertaken to elucidate the mechanism of Th2-mediated destruction of B16-F1 melanoma cells by examining the in vitro antitumor activity of leukocytes within a type-2 inflammatory infiltrate. The experimental data show that activation of alternatively activated macrophages (aaMacs) within type-2 infiltrates by IL-4 or IL-13 can inhibit B16-F1 melanoma cell proliferation through a mechanism that is dependent on arginase-1 depletion of L-arginine within the tumor cell microenvironment. Interestingly, whilst at higher E:T ratios aaMac exhibited antitumor activity, at lower E:T ratios aaMacs were observed to enhance rather than inhibit B16-F1 melanoma cell growth. This highlights the fine balance between stimulating the antitumorigenic and protumorigenic properties of aaMacs in tumor immunotherapy protocols.
dc.publisherLippincott Williams & Wilkins
dc.sourceJournal of Immunotherapy
dc.subjectKeywords: arginase 1; arginine; CD4 antigen; interleukin 13; interleukin 4; adoptive transfer; animal cell; antigen specificity; antineoplastic activity; article; cancer immunotherapy; CD4+ T lymphocyte; cell destruction; cell growth; cell proliferation; controlled Cytotoxicity; Interleukin-4; Monocytes/macrophages; Tumor immunity
dc.titleAlternatively activated macrophage possess antitumor cytotoxicity that is induced by il-4 and mediated by arginase-1
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume33
dc.date.issued2010
local.identifier.absfor110799 - Immunology not elsewhere classified
local.identifier.ariespublicationf2965xPUB815
local.type.statusPublished Version
local.contributor.affiliationEllyard, Julia, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationQuah, Ben, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSimson, Ljubov, University of Canberra
local.contributor.affiliationParish, Christopher, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage443
local.bibliographicCitation.lastpage452
local.identifier.doi10.1097/CJI.0b013e3181cd8746
local.identifier.absseo920102 - Cancer and Related Disorders
local.identifier.absseo920108 - Immune System and Allergy
dc.date.updated2016-02-24T08:33:07Z
local.identifier.scopusID2-s2.0-77953033498
local.identifier.thomsonID000278104600001
CollectionsANU Research Publications

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