Shelden, Megan; Dong, B; de Bruxelles, G; Trevaskis, B; Whelan, James M; Ryan, Peter; Howitt, Susan; Udvardi, Michael K
We have compared the biochemical properties of two different Arabidopsis ammonium transporters, AtAMT1;1 and AtAMT1;2, expressed in yeast, with the biophysical properties of ammonium transport in planta. Expression of the AtAMT1;1 gene in Arabidopsis roots increased approximately four-fold in response to nitrogen deprivation. This coincided with a similar increase in high-affinity ammonium uptake by these plants. The biophysical characteristics of this high-affinity system (Km for ammonium and...[Show more]
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