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The thioflavin T fluorescence assay for amyloid fibril detection can be biased by the presence of exogenous compounds

Hudson, Sean; Ecroyd, Heath; Kee, Tak W; Carver, John


Thioflavin T (ThT) dye fluorescence is used regularly to quantify the formation and inhibition of amyloid fibrils in the presence of anti-amyloidogenic compounds such as polyphenols. However, in this study, it was shown, using three polyphenolics (curcumin, quercetin and resveratrol), that ThT fluorescence should be used with caution in the presence of such exogenous compounds. The strong absorptive and fluorescent properties of quercetin and curcumin were found to significantly bias the ThT...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
Source: The FEBS Journal
DOI: 10.1111/j.1742-4658.2009.07307.x


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