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Implantation of functionalized thermally gelling xyloglucan hydrogel within the brain: associated neurite infiltration and inflammatory response

Nisbet, David; Rodda, AE; Horne, M; Forsythe, John S.; Finkelstein, D I

Description

To develop neural tissue engineering strategies that are useful for repairing damaged neural pathways in the central nervous system, it is essential to control and optimise neurone and neurite interactions with functional scaffolds. In this study, the suitability of thermally gelling xyloglucan hydrogels, along with xyloglucan-graft-poly-d-lysine (PDL) hydrogels, was assessed through their implantation within the caudate putamen of adult rats. The ability of the hydrogel scaffolds to encourage...[Show more]

dc.contributor.authorNisbet, David
dc.contributor.authorRodda, AE
dc.contributor.authorHorne, M
dc.contributor.authorForsythe, John S.
dc.contributor.authorFinkelstein, D I
dc.date.accessioned2015-12-10T23:08:01Z
dc.identifier.issn1937-3341
dc.identifier.urihttp://hdl.handle.net/1885/63116
dc.description.abstractTo develop neural tissue engineering strategies that are useful for repairing damaged neural pathways in the central nervous system, it is essential to control and optimise neurone and neurite interactions with functional scaffolds. In this study, the suitability of thermally gelling xyloglucan hydrogels, along with xyloglucan-graft-poly-d-lysine (PDL) hydrogels, was assessed through their implantation within the caudate putamen of adult rats. The ability of the hydrogel scaffolds to encourage the infiltration of axons in a controlled manner was investigated, as was the inflammatory response associated with the implantation. The microglia reaction was the same for unmodified xyloglucan and the xyloglucan-graft-PDL scaffolds, peaking after 3 days before decreasing back to homeostatic levels after ∼28 days. Penetration of the microglia into the scaffold was not observed, with these cells accumulating at the scaffold-tissue interface. For astrocytes, the other type of glial cell with migratory capacity, the peak activation occurred between 14 and 21 days. This reaction subsided more rapidly for the unmodified scaffold compared to the xyloglucan-graft-PDL scaffolds, which remained elevated 21-28 days before returning to homeostatic levels within 60 days. Most noteworthy was the discovery of increased infiltration levels for astrocytes and neurites with higher concentrations of grafted PDL. The timing of the astrocyte migration coincided with neurite infiltration within the scaffolds, suggesting that astrocytes may have facilitated this infiltration, possibly due to the secretion of laminin.
dc.publisherMary Ann Liebert Inc.
dc.sourceTissue Engineering Part A: Tissue Engineering
dc.subjectKeywords: Central nervous systems; Functionalized; Glial cells; Hydrogel scaffolds; Inflammatory response; Laminin; Microglia; Neural pathway; Neural tissue engineering; Neurites; Poly d lysines; Thermally gelling; Tissue interface; Xyloglucans; Amino acids; Gelati
dc.titleImplantation of functionalized thermally gelling xyloglucan hydrogel within the brain: associated neurite infiltration and inflammatory response
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume16
dc.date.issued2010
local.identifier.absfor090399 - Biomedical Engineering not elsewhere classified
local.identifier.ariespublicationu4334215xPUB771
local.type.statusPublished Version
local.contributor.affiliationNisbet, David, College of Engineering and Computer Science, ANU
local.contributor.affiliationRodda, AE, Monash University
local.contributor.affiliationHorne, M, Monash University
local.contributor.affiliationForsythe, John S., Monash University
local.contributor.affiliationFinkelstein, D, Monash University
local.description.embargo2037-12-31
local.bibliographicCitation.issue9
local.bibliographicCitation.startpage2833
local.bibliographicCitation.lastpage42
local.identifier.doi10.1089/ten.tea.2009.0677
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.absseo970109 - Expanding Knowledge in Engineering
dc.date.updated2016-02-24T11:02:58Z
local.identifier.scopusID2-s2.0-77956085517
local.identifier.thomsonID000281288500012
CollectionsANU Research Publications

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