Exposing local adaptation: synergistic stressors elicit population-specific lactate dehydrogenase-B (ldh-b) expression profiles in Australian barramundi, Lates calcarifer
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Edmunds, Richard; Smith-Keune, Carolyn; van Herwerden, Lynne; Fulton, Christopher; Jerry, Dean R.
Description
The molecular response of fish to independently and/or concurrently applied ecological stressors (e. g. thermal and/or aerobic stress) can be quantified at the level of transcript abundance (i. e. gene expression). In temperate fish, the expression of the metabolic candidate gene lactate dehydrogenase-B (ldh-b) responds to both aerobic swimming challenge and extended acclimation to various ecologically relevant temperatures. We examined hepatic ldh-b expression in juvenile Lates calcarifer from...[Show more]
dc.contributor.author | Edmunds, Richard | |
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dc.contributor.author | Smith-Keune, Carolyn | |
dc.contributor.author | van Herwerden, Lynne | |
dc.contributor.author | Fulton, Christopher | |
dc.contributor.author | Jerry, Dean R. | |
dc.date.accessioned | 2015-12-10T23:07:45Z | |
dc.identifier.issn | 1015-1621 | |
dc.identifier.uri | http://hdl.handle.net/1885/62996 | |
dc.description.abstract | The molecular response of fish to independently and/or concurrently applied ecological stressors (e. g. thermal and/or aerobic stress) can be quantified at the level of transcript abundance (i. e. gene expression). In temperate fish, the expression of the metabolic candidate gene lactate dehydrogenase-B (ldh-b) responds to both aerobic swimming challenge and extended acclimation to various ecologically relevant temperatures. We examined hepatic ldh-b expression in juvenile Lates calcarifer from two geographically, genetically and thermally distinct Australian populations to determine if similar environmental stressors also influence the transcription of this locus in a tropical fish. Hepatic ldh-b expression was quantified following 28-day acclimation to ecologically relevant temperatures (20, 25, 30 and 35°C). Expression was also quantified in L. calcarifer subjected to aerobic swimming challenge at these temperatures. Fish from southern (high latitude) and northern (low latitude) populations within this species' Australian distribution exhibited a significant increase in hepatic ldh-b expression following aerobic swimming challenge at native temperatures of 25 and 30°C, respectively (p < 0.001). Southern and northern fish also exhibited significant increase in hepatic ldh-b expression (p < 0.001 and p < 0.01, respectively) following 28-day acclimation to heat-stress (35°C). However, only southern fish exhibited significant increase in expression (p < 0.001) following 28-day acclimation to cold-stress (20°C). The novel evidence presented herein suggests that (a) transcription of hepatic ldh-b is responsive to both aerobic and thermal stress when applied independently, and (b) southern Australian L. calcarifer populations may be locally adapted to cooler seasonal water temperatures. | |
dc.publisher | Birkhauser Verlag | |
dc.source | Aquatic Sciences | |
dc.subject | Keywords: acclimation; environmental stress; enzyme activity; fish; gene expression; latitude; local adaptation; molecular analysis; physiological response; population structure; temperate environment; temperature effect; tolerance; tropical environment; Australia; Aerobic challenge; Local adaptation; Thermal stress; Thermal tolerance | |
dc.title | Exposing local adaptation: synergistic stressors elicit population-specific lactate dehydrogenase-B (ldh-b) expression profiles in Australian barramundi, Lates calcarifer | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | online 21 May 2011 | |
dc.date.issued | 2011 | |
local.identifier.absfor | 060107 - Enzymes | |
local.identifier.absfor | 060203 - Ecological Physiology | |
local.identifier.ariespublication | u9511635xPUB758 | |
local.type.status | Published Version | |
local.contributor.affiliation | Edmunds, Richard, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Smith-Keune, Carolyn, James Cook University | |
local.contributor.affiliation | van Herwerden, Lynne, James Cook University | |
local.contributor.affiliation | Fulton, Christopher, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Jerry, Dean R., James Cook University | |
local.description.embargo | 2037-12-31 | |
local.identifier.doi | 10.1007/s00027-011-0208-5 | |
local.identifier.absseo | 960301 - Climate Change Adaptation Measures | |
local.identifier.absseo | 960307 - Effects of Climate Change and Variability on Australia (excl. Social Impacts) | |
local.identifier.absseo | 830102 - Aquaculture Fin Fish (excl. Tuna) | |
dc.date.updated | 2016-02-24T12:08:20Z | |
local.identifier.scopusID | 2-s2.0-84855302141 | |
local.identifier.thomsonID | 000298750900014 | |
Collections | ANU Research Publications |
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